GMDS Polyclonal antibody

GMDS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15442-1-AP

Synonyms

SDR3E1, GMD, GDP-mannose 4,6 dehydratase, GDP-D-mannose dehydratase, EC:4.2.1.47

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Validation Data Gallery

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  WB analysis using 15442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15442-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15442-1-AP (GMDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15442-1-AP (GMDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15442-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15442-1-AP (GMDS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15442-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15442-1-AP (GMDS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, HeLa cells, rat spleen tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

15442-1-AP targets GMDS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMDS fusion protein Ag7319
• Full Name: GDP-mannose 4,6-dehydratase
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC000117
• Gene Symbol: GMDS
• Gene ID (NCBI): 2762
• RRID: AB_2110816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the 'de novo' pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor.

Publications

Species	Application	Title
human	WB,IP	Mol Cell Biol; GDP-mannose-4,6-dehydratase is a cytosolic partner of tankyrase 1 that inhibits its poly(ADP-ribose) polymerase activity.; Authors - Bisht Kamlesh K KK
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.; Authors - Lilli Saarinen
human	WB	Mol Cell Proteomics; Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei; Authors - Lilli Saarinen
human	WB	Cell Chem Biol; High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery.; Authors - Yasuhiko Kizuka