GMEB2 Polyclonal antibody

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13314-1-AP

Synonyms

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

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Validation Data Gallery

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  WB analysis of human placenta using 13314-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13314-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 13314-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 13314-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 13314-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 13314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse ovary tissue, mouse thymus tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13314-1-AP targets GMEB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GMEB2 fusion protein Ag4157
• Full Name: glucocorticoid modulatory element binding protein 2
• Calculated Molecular Weight: 530 aa, 56 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC036305
• Gene Symbol: GMEB2
• Gene ID (NCBI): 26205
• RRID: AB_2110829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.