Tested Applications
Positive WB detected in | Jurkat cells |
Positive IF/ICC detected in | K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86101-2-RR targets GMIP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | GMIP fusion protein Ag23239 Predict reactive species |
Full Name | GEM interacting protein |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC126436 |
Gene Symbol | GMIP |
Gene ID (NCBI) | 51291 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9P107 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.
Protocols
Product Specific Protocols | |
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WB protocol for GMIP antibody 86101-2-RR | Download protocol |
IF protocol for GMIP antibody 86101-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |