GMIP Recombinant antibody

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  WB analysis using 86101-2-RR

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 86101-2-RR (GMIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 86101-2-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GMIP antibody (86101-2-RR, Clone: 250483G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GMIP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86101-2-RR

Clone No.250483G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ARHGAP46, GEM interacting protein, GEM-interacting protein

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86101-2-RR targets GMIP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GMIP fusion protein Ag23239
• Full Name: GEM interacting protein
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC126436
• Gene Symbol: GMIP
• Gene ID (NCBI): 51291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9P107
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.