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GNA13 Monoclonal antibody

GNA13 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2G8F10

Cat no : 67188-1-Ig

Synonyms

G alpha 13, G protein subunit alpha 13, G13, GNA13



Tested Applications

Positive WB detected inHEK-293 cells, Y79 cells, HepG2 cells, PC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

67188-1-Ig targets GNA13 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNA13 fusion protein Ag7316
Full Name guanine nucleotide binding protein (G protein), alpha 13
Calculated molecular weight 44 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC036756
Gene symbol GNA13
Gene ID (NCBI) 10672
RRIDAB_2882483
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GNA13 antibody 67188-1-IgDownload protocol
IHC protocol for GNA13 antibody 67188-1-IgDownload protocol
IF protocol for GNA13 antibody 67188-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis

Authors - Yaping Zhang
humanWB,CoIP

J Proteome Res

Proteomic Investigation of Differential Interactomes of Glypican 1 and a Putative Disease-Modifying Variant of Ataxia

Authors - Nur Cengiz Winter

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (12-29-2023)

Worked well for WB at 1:1000 in Glioma cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioma