GNA13 Monoclonal antibody, PBS Only

• 

  WB analysis using 67188-1-Ig (same clone as 67188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67188-1-Ig (GNA13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67188-1-Ig (same clone as 67188-1-PBS)

  WB result of GNA13 antibody (67188-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNA13 transfected HEK-293 cells. This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67188-1-Ig (same clone as 67188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67188-1-Ig (same clone as 67188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67188-1-Ig (same clone as 67188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67188-1-Ig (same clone as 67188-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67188-1-Ig (same clone as 67188-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNA13 antibody (67188-1-Ig, Clone: 2G8F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GNA13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67188-1-PBS

Clone No.2G8F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

G alpha 13, G alpha-13, G protein subunit alpha 13, G13, G-protein subunit alpha-13

---

Product Information

67188-1-PBS targets GNA13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7316

  Product name: Recombinant human GNA13 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-377 aa of BC036756

  Sequence: MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ

• Full Name: guanine nucleotide binding protein (G protein), alpha 13
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC036756
• Gene Symbol: GNA13
• Gene ID (NCBI): 10672
• RRID: AB_2882483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q14344
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GNA13 (G Protein Subunit Alpha 13) is a protein-coding gene located on chromosome 17. It encodes the alpha subunit of the heterotrimeric G protein complex, which acts as a molecular switch to transmit signals from cell surface receptors to the interior of the cell. Under calorie restriction, GNA13 expression is dynamically regulated, thereby influencing alternative splicing-particularly in genes downstream of the G protein-coupled receptor (GPCR) signaling pathway-and contributing to the modulation of aging-related processes.