GNL1 Polyclonal antibody

GNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14078-1-AP

Synonyms

HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

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Validation Data Gallery

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  WB analysis using 14078-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14078-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 14078-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14078-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14078-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14078-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNL1 antibody (14078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 14078-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GNL1 antibody 14078-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Raji cells, U-937 cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human heart tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14078-1-AP targets GNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNL1 fusion protein Ag5203
• Full Name: guanine nucleotide binding protein-like 1
• Calculated Molecular Weight: 607 aa, 69 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC018366
• Gene Symbol: GNL1
• Gene ID (NCBI): 2794
• RRID: AB_10641989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Guanine nucleotide-binding protein-like 1 (GNL1), also known as HSR1, belongs to HSR1_MMR1 subfamily of nucleolar GTPases. It encodes 607 amino acids with a molecular mass of 69 kDa and contains basic amino acids rich N-terminus, acidic amino acids rich C-terminus and proline rich-domains. GNL1 plays a critical role in liver cell proliferation, but its function remains largely unknown (PMID: 18255255).

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu