GNL2 Polyclonal antibody

GNL2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10190-2-AP

Synonyms

Autoantigen NGP 1, GNL2, HUMAUANTIG, Ngp 1, NGP1

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Validation Data Gallery

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  WB analysis using 10190-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10190-2-AP (GNL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10190-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10190-2-AP (GNL2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Ramos cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10190-2-AP targets GNL2 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNL2 fusion protein Ag0243
• Full Name: guanine nucleotide binding protein-like 2 (nucleolar)
• Calculated Molecular Weight: 731 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC000107
• Gene Symbol: GNL2
• Gene ID (NCBI): 29889
• RRID: AB_2110075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.