GNL3 Monoclonal antibody

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  WB analysis using 67169-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67169-1-Ig

  Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

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  IF Staining of HeLa using 67169-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L).

GNL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67169-1-Ig

Clone No.3B8F10

Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

67169-1-Ig targets GNL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7056

  Product name: Recombinant human GNL3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 201-549 aa of BC001024

  Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

• Full Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC001024
• Gene Symbol: GNL3
• Gene ID (NCBI): 26354
• RRID: AB_2882465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BVP2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Int J Oral Sci; Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC; Authors - Lixuan Wang
human,mouse	WB	Science; A subcellular map of translational machinery composition and regulation at the single-molecule level; Authors - Zijian Zhang