GNL3 Monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 67169-1-Ig (same clone as 67169-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

  Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP51635-2

  Cytometric bead array sample test of MP51635-2, GNL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67169-4-PBS. Detection antibody: 67169-1-PBS.

• 

  Cytometric bead array standard curve of MP51635-2

  Cytometric bead array standard curve of MP51635-2, GNL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67169-4-PBS. Detection antibody: 67169-1-PBS. Standard:Ag7056. Range: 1.563-200 ng/mL.

GNL3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67169-1-PBS

Clone No.3B8F10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

3B8F10, C77032, E2 induced gene 3 protein, E2IG3, E2-induced gene 3 protein

---

Product Information

67169-1-PBS targets GNL3 as part of a matched antibody pair:

MP51635-2: 67169-4-PBS capture and 67169-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7056

  Product name: Recombinant human GNL3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 201-549 aa of BC001024

  Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

• Full Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC001024
• Gene Symbol: GNL3
• Gene ID (NCBI): 26354
• RRID: AB_2882465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BVP2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.