GNL3 Monoclonal antibody, PBS Only

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  WB analysis using 67169-1-Ig (same clone as 67169-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

  Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

GNL3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67169-1-PBS

Clone No.3B8F10

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

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Product Information

67169-1-PBS targets GNL3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7056

  Product name: Recombinant human GNL3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 201-549 aa of BC001024

  Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

• Full Name: guanine nucleotide binding protein-like 3 (nucleolar)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC001024
• Gene Symbol: GNL3
• Gene ID (NCBI): 26354
• RRID: AB_2882465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BVP2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.