GNPDA1 Polyclonal antibody

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GNPDA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12312-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

GlcN6P deaminase 1, GNPDA, GNPDA 1, GNPDA1, GNPI, GPI, HLN, KIAA0060, Oscillin

• 

  WB analysis of K-562 using 12312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 12312-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 12312-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 12312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12312-1-AP (GNPDA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 12312-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12312-1-AP (GNPDA1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, human brain tissue, rat kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12312-1-AP targets GNPDA1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2961

  Product name: Recombinant human GNPDA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-289 aa of BC012853

  Sequence: MKLIILEHYSQASEWAAKYIRNRIIQFNPGPEKYFTLGLPTGSTPLGCYKKLIEYYKNGDLSFKYVKTFNMDEYVGLPRDHPESYHSFMWNNFFKHIDIHPENTHILDGNAVDLQAECDAFEEKIKAAGGIELFVGGIGPDGHIAFNEPGSSLVSRTRVKTLAMDTILANARFFDGELTKVPTMALTVGVGTVMDAREVMILITGAHKAFALYKAIEEGVNHMWTVSAFQQHPRTVFVCDEDATLELKVKTVKYFKGLMLVHNKLVDPLYSIKEKETEKSQSSKKPYSD

• Full Name: glucosamine-6-phosphate deaminase 1
• Calculated Molecular Weight: 289 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC012853
• Gene Symbol: GNPDA1
• Gene ID (NCBI): 10007
• RRID: AB_2110553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46926
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).

Publications

What published studies show

The GNPDA1 Polyclonal antibody (Cat# 12312-1-AP) has 5 citations published in Frontiers in Cell and Developmental Biology, iScience, Glycobiology, Discover Oncology, and Cancer Management Research. The associated research fields include hepatocellular carcinoma, bladder cancer, keratinocyte hexosamine biosynthesis, tongue squamous cell carcinoma, and tumor progression/prognosis in HCC.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Cell Dev Biol; Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.; Authors - Yajuan Zhao
human	WB,IP	iScience; RBM11 drives malignant progression of bladder cancer by regulating GNPDA1-PKM2 axis.; Authors - Hang Tong
human	WB	Glycobiology; Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.; Authors - Sanna Oikari ; KD Validated
human	WB	Discov Oncol; Tongue squamous cell carcinoma exosomal miR-122-5p promotes the conversion from fibroblasts to inflammatory cancer-associated fibroblasts.; Authors - Bin Xia
human	WB	Cancer Manag Res; Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.; Authors - Dezhi Li ; KD Validated