CoraLite® Plus 555-conjugated GOLGA2/GM130 Recombinant antibody

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  IF Staining of HeLa using CL555-82705-8

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 555 GOLGA2/GM130 antibody (CL555-82705-8, Clone: 1M17 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Recombinant Antibody for IF/ICC

Cat No. CL555-82705-8

Clone No.1M17

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Synonyms

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, 1M17, GM130 autoantigen

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-82705-8 targets GOLGA2/GM130 in IF/ICC applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08379
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)