GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6C6

Cat no : 67738-1-Ig

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A



Tested Applications

Positive WB detected inpig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse heart tissue, rat brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Sample-dependent, check data in validation data gallery

Product Information

67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

Tested Reactivity Human, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT2 fusion protein Ag6600
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated molecular weight 47 kDa
Observed molecular weight39-40 kDa
GenBank accession numberBC000525
Gene symbol GOT2
Gene ID (NCBI) 2806
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Protocols

Product Specific Protocols
WB protocol for GOT2 antibody 67738-1-IgDownload protocol
IHC protocol for GOT2 antibody 67738-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols