GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6C6

Cat no : 67738-1-Ig

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

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Validation Data Gallery

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  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67738-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67738-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells
• Positive IHC detected in: mouse heart tissue, rat brain tissue, rat heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

Sample-dependent, check data in validation data gallery

Product Information

67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

• Tested Reactivity: Human, mouse, rat, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOT2 fusion protein Ag6600
• Full Name: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 39-40 kDa
• GenBank accession number: BC000525
• Gene symbol: GOT2
• Gene ID (NCBI): 2806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.