GPAA1 Polyclonal antibody, PBS Only

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• GPAA1 Polyclonal antibody, PBS Only (10104-1-PBS)

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  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 10104-1-AP (same clone as 10104-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10104-1-AP (GPAA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10104-1-AP (same clone as 10104-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10104-1-AP (GPAA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10104-1-PBS in a different storage buffer formulation.

GPAA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 10104-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GAA1, Glycosylphosphatidylinositol anchor attachment 1 protein, GPAA 1, hGAA1

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Product Information

10104-1-PBS targets GPAA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0152

  Product name: Recombinant human GPAA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 62-321 aa of BC004129

  Sequence: EQFAGGDRARAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAASTESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSSPLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSLDGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRG

• Full Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC004129
• Gene Symbol: GPAA1
• Gene ID (NCBI): 8733
• RRID: AB_2263708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43292
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.