GPD1L Polyclonal antibody

• 

  WB analysis of mouse heart using 17263-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 17263-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17263-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17263-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17263-1-AP (GPD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

GPD1L Polyclonal Antibody for WB, ELISA

Cat No. 17263-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GPD1-L, GPD1 L, Glycerol-3-phosphate dehydrogenase 1-like protein, EC:1.1.1.8

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Tested Applications

• Positive WB detected in: mouse heart tissue, human skeletal muscle tissue, human brain tissue, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

17263-1-AP targets GPD1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10977

  Product name: Recombinant human GPD1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC028726

  Sequence: MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEHT

• Full Name: glycerol-3-phosphate dehydrogenase 1-like
• Calculated Molecular Weight: 351 aa, 38 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC028726
• Gene Symbol: GPD1L
• Gene ID (NCBI): 23171
• RRID: AB_2112359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N335
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease.; Authors - Shanshan Liu
human	WB	Proc Natl Acad Sci U S A; A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria.; Authors - Shiqi Wu
rat	WB	J Transl Med; Atrial myocyte-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation; Authors - Hongting Hao
human	WB	Cell Death Dis; A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer.; Authors - Ye Du
human,mouse	WB	Mol Cell; Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis; Authors - Cong-Hui Yao ; KD Validated
human	WB	Nat Cell Biol; AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress; Authors - Xuewei Zhai