GPR137 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR137 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11929-1-AP

Synonyms

C11orf4, G protein coupled receptor 137, GPR137, GPR137A, TM7SF1L1

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Validation Data Gallery

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  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11929-1-AP (GPR137 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11929-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11929-1-AP (GPR137 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11929-1-AP targets GPR137 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR137 fusion protein Ag2611
• Full Name: G protein-coupled receptor 137
• Calculated Molecular Weight: 396 aa, 44 kDa
• GenBank Accession Number: BC033920
• Gene Symbol: GPR137
• Gene ID (NCBI): 56834
• RRID: AB_10694423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G protein-coupled receptors (GPRs) are highly related to oncogenesis and cancer metastasis. G protein-coupled receptor 137 (GPR137) was initially reported as a novel orphan GPR about 10 years ago. GPR137 is ubiquitously expressed in the central nervous system (CNS), mainly in the hippocampus (PMID: 22025619). GPR137 is involved in the progression of human glioma, suggesting GPR137 as a potential oncogene of glioma cells (PMID: 24870220). Recent study shows that GPR137 plays an important role in colon cancer cell proliferation (PMID: 25301753).

Publications

Species	Application	Title
human	WB	Biotechnol Appl Biochem; Knockdown of GPR137 by RNAi inhibits pancreatic cancer cell growth and induces apoptosis.; Authors - Xianping Cui
human	WB, IF	Biotechnol Appl Biochem; G-protein-coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro.; Authors - Yiheng Du ; KD Validated
human	WB	Cell Biol Int; Down-Regulation of G Protein-Coupled Receptor 137 by RNA Interference Inhibits Cell Growth of Two Hepatoma Cell Lines.; Authors - Xin Shao
human	IHC	Neurol Sci; Inhibition of GPR137 expression reduces the proliferation and colony formation of malignant glioma cells.; Authors - Gang Zong
human	WB	J Bone Oncol; Small interfering RNA-mediated silencing of G-protein-coupled receptor 137 inhibits growth of osteosarcoma cells.; Authors - Hao Li
human	WB	Mol Med Rep; RNA interference-mediated silencing of G protein-coupled receptor 137 inhibits human gastric cancer cell growth.; Authors - Zishu Wang ; KD Validated