GPR142 Polyclonal antibody

GPR142 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18820-1-AP

Synonyms

G protein coupled receptor 142, GPR142, PGR2

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 18820-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18820-1-AP (GPR142 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18820-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18820-1-AP (GPR142 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 18820-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug GPR142 antibody (18820-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18820-1-AP targets GPR142 in WB, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 142
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC152987
• Gene Symbol: GPR142
• Gene ID (NCBI): 350383
• RRID: AB_10597384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR142, also named as PGR2, belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody recognizes the 51-kDa GRP142.

Publications

Species	Application	Title
	WB	BMC Genomics; The fiber diameter traits of Tibetan cashmere goats are governed by the inherent differences in stress, hypoxic, and metabolic adaptations: an integrative study of proteome and transcriptome.; Authors - Bingru Zhao