GPR150-Specific Polyclonal antibody

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  IHC staining of mouse brain using 20213-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20213-1-AP (GPR150-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 20213-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GPR150-Specific antibody (20213-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

GPR150-Specific Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 20213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

GPR150, Probable G-protein coupled receptor 150, PGR11, G protein coupled receptor 150

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20213-1-AP targets GPR150-Specific in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 150
• Calculated Molecular Weight: 46 kDa
• GenBank Accession Number: NM_199243
• Gene Symbol: GPR150
• Gene ID (NCBI): 285601
• RRID: AB_3669330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NGU9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR150 belongs to the G-protein coupled receptor 1 family. It is an orphan receptor. This antibody is specific to GPR150.