GPR35 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR35 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55248-1-AP

Synonyms

Kynurenic acid receptor, KYNA receptor, G-protein coupled receptor 35, G protein coupled receptor 35

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Validation Data Gallery

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  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55248-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 55248-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55248-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 55248-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55248-1-AP (GPR35 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 55248-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55248-1-AP (GPR35 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, human stomach tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

55248-1-AP targets GPR35 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: G protein-coupled receptor 35
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: NM_005301
• Gene Symbol: GPR35
• Gene ID (NCBI): 2859
• RRID: AB_10836935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR35, also named the KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Publications

Species	Application	Title
human	WB	Acta Pharm Sin B; Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.; Authors - Di Wang ; KD Validated
human	WB	Eur J Pharmacol; Aminosalicylates target GPR35, partly contributing to the prevention of DSS-induced colitis; Authors - Wuxiyar Otkur
human	WB	Science; Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists; Authors - Gregory A Wyant