GPR56 Polyclonal antibody

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  WB analysis using 32532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32532-1-AP (GPR56 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GPR56 Polyclonal Antibody for WB, ELISA

Cat No. 32532-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ADGRG1, ADGRG1 CT, ADGRG1 C-terminal fragment, ADGRG1 NT, ADGRG1 N-terminal fragment

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Tested Applications

• Positive WB detected in: A549 cells, Calu-1 cells, K-562 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32532-1-AP targets GPR56 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: G protein-coupled receptor 56
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_001145771.2
• Gene Symbol: GPR56
• Gene ID (NCBI): 9289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y653
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR56, also known as ADGRG1, is a member of the adhesion G protein-coupled receptor (aGPCR) family. These receptors are characterized by an extended extracellular region with a GPCR autoproteolysis inducing (GAIN) domain (PMID: 37980704). GPR56 is widely expressed in various tissues, including the liver, muscle, tendon, neural cells, and cytotoxic lymphoid cells in humans. It plays crucial roles in multiple biological processes such as cell guidance, adhesion, and development.