GPRC5D Polyclonal antibody

GPRC5D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21089-1-AP

Synonyms

G-protein coupled receptor family C group 5 member D

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Validation Data Gallery

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  WB analysis using 21089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21089-1-AP (GPRC5D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21089-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 21089-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21089-1-AP (GPRC5D Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, THP-1 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21089-1-AP targets GPRC5D in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPRC5D fusion protein Ag15338
• Full Name: G protein-coupled receptor, family C, group 5, member D
• Calculated Molecular Weight: 345 aa, 39 kDa
• Observed Molecular Weight: 34-39 kDa
• GenBank Accession Number: BC069341
• Gene Symbol: GPRC5D
• Gene ID (NCBI): 55507
• RRID: AB_2878810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.