GPRIN3-Specific Polyclonal antibody

GPRIN3-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20212-1-AP

Synonyms

GPRIN3, KIAA2027, GRIN3, GPRIN family member 3, G protein-regulated inducer of neurite outgrowth 3

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Validation Data Gallery

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  WB analysis of human brain using 20212-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20212-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20212-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20212-1-AP (GPRIN3-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20212-1-AP targets GPRIN3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: GPRIN family member 3
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: NM_198281
• Gene Symbol: GPRIN3
• Gene ID (NCBI): 285513
• RRID: AB_10666200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPRIN3, also named as KIAA2027, may be involved in neurite outgrowth. The antibody is specific to GPRIN3.

Publications

Species	Application	Title
mouse	WB	Mol Cell Biol; UBE3D Regulates mRNA 3\'-End Processing and Maintains Adipogenic Potential in 3T3-L1 Cells; Authors - Daniel Heller-Trulli