CSN1 Polyclonal antibody

CSN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11709-1-AP

Synonyms

GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1

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Validation Data Gallery

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  WB analysis using 11709-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11709-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11709-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 11709-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 11709-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11709-1-AP

  IP result of anti-CSN1 (IP:11709-1-AP, 4ug; Detection:11709-1-AP 1:500) with HeLa cells lysate 2600 ug.

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  IHC staining of human prostate cancer using 11709-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11709-1-AP (CSN1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11709-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11709-1-AP (CSN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, HT-1080 cells, Raji cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11709-1-AP targets CSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSN1 fusion protein Ag2273
• Full Name: G protein pathway suppressor 1
• Calculated Molecular Weight: 491 aa, 55-60 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC000155
• Gene Symbol: CSN1
• Gene ID (NCBI): 2873
• RRID: AB_2232685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Med Rep; CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.; Authors - Hangwei Fu