GPSM2 Recombinant antibody

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  WB analysis using 86175-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86175-1-RR (GPSM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GPSM2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86175-1-RR

Clone No.250793G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

G protein signaling modulator 2, G-protein-signaling modulator 2, LGN, Pins

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86175-1-RR targets GPSM2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPSM2 fusion protein Ag25195
• Full Name: G-protein signaling modulator 2 (AGS3-like, C. elegans)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-77 kDa
• GenBank Accession Number: BC027732
• Gene Symbol: GPSM2
• Gene ID (NCBI): 29899
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P81274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPSM2 belongs to a family of proteins that modulate activation of G proteins. GPSM2 assists in the exchange of guanine nucleotides, and allows extracellular signals to be transmitted to cells via cell surface, and ultimately plays a key role in the activation of G-proteins. Therefore, GPSM2 is a critical factor for the stability of cell division. Some recent studies have shown that GPSM2 messenger RNA (mRNA) is overexpressed and plays a positive role in the development of certain tumors, such as liver cancer, pancreatic cancer, breast cancer. It also plays a role in neuroblast division and in the development of normal hearing. Mutations in GPSM2 are associated with autosomal recessive nonsyndromic deafness (DFNB82), which is a form of non-syndromic deafness characterized by prelingual, bilateral, severe, sensorineural hearing loss.