GPX5 Polyclonal antibody, PBS Only

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• GPX5 Polyclonal antibody, PBS Only (18731-1-PBS)

• 

  WB analysis of mouse testis using 18731-1-AP (same clone as 18731-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18731-1-AP (same clone as 18731-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 18731-1-AP (same clone as 18731-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 18731-1-AP (same clone as 18731-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18731-1-AP (GPX5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18731-1-PBS in a different storage buffer formulation.

GPX5 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 18731-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

EC:1.11.1.9, EGLP, Epididymis-specific glutathione peroxidase-like protein, GPx 5, GPx-5

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Product Information

18731-1-PBS targets GPX5 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: glutathione peroxidase 5 (epididymal androgen-related protein)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 22-28 kDa
• GenBank Accession Number: NM_001509
• Gene Symbol: GPX5
• Gene ID (NCBI): 2880
• RRID: AB_10596913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75715
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GPX5 belongs to the glutathione peroxidase family. GPX5 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Catalytic the reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. GPX5 constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids. GPX5 has a calculated molecular mass of 25 kDa and apparent molecular mass of 22-28 kDa due to the cleavage of signal peptide and glycosylation.