Tested Applications
| Positive WB detected in | HeLa cells, HSC-T6 cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SK-BR-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1938-1:7752 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66651-1-Ig targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1054 Product name: Recombinant human GRASP65,GORASP1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-215 aa of BC008928 Sequence: MGLGVSAEQPAGGAEGFHLHGVQENSPAQQAGLEPYFDFIITIGHSRLNKENDTLKALLKANVEKPVKLEVFNMKTMRVREVEVVPSNMWGGQGLLGASVRFCSFRRASEQVWHVLDVEPSSPAALAGLRPYTDYVVGSDQILQESEDFFTLIESHEGKPLKLMVYNSKSDSCRESGMWHWLWVSTPDPNSAPQLPQEATWHPTTFCSTTWCPTT Predict reactive species |
| Full Name | golgi reassembly stacking protein 1, 65kDa |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC008928 |
| Gene Symbol | GRASP65 |
| Gene ID (NCBI) | 64689 |
| RRID | AB_2882008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9BQQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| IHC protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| WB protocol for GRASP65,GORASP1 antibody 66651-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRASP65 (GORASP1) Monoclonal antibody (1B2C10), catalog number 66651-1-Ig, has 4 total citations published in Cells, Frontiers in Cell and Developmental Biology, Molecular Biology of the Cell, and Journal of Cellular Biochemistry. The associated research fields span secretory autophagy and wound healing, AIM2 inflammasome-mediated DNA damage resistance, Golgi-mediated protein trafficking, and oxidized LDL-induced endothelial cell injury, with all studies utilizing Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cells Discovery of Cell Number-Interstitial Fluid Volume (CIF) Ratio Reveals Secretory Autophagy Pathway to Supply eHsp90α for Wound Healing
| ||
Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
J Cell Biochem Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury. |
