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GRB2 Polyclonal antibody

GRB2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 10254-2-AP


Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, rat spleen tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10254-2-AP targets GRB2 in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRB2 fusion protein Ag0395
Full Name growth factor receptor-bound protein 2
Calculated molecular weight 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC000631
Gene symbol GRB2
Gene ID (NCBI) 2885
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.


Product Specific Protocols
WB protocol for GRB2 antibody 10254-2-APDownload protocol
IHC protocol for GRB2 antibody 10254-2-APDownload protocol
IF protocol for GRB2 antibody 10254-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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