GRB2 Recombinant monoclonal antibody, PBS Only (Capture)

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• GRB2 Recombinant monoclonal antibody, PBS Only (Capture) (88060-1-PBS)

GRB2 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 88060-1-PBS

Clone No.260129D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2

• 

  WB analysis using 88060-1-RR (same clone as 88060-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88060-1-RR (GRB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88060-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03345-1

  The mean GRB2 concentration was determined to be 16.3 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 8.2 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load, 0.6 ng/mL in HaCaT cell extract based on a 1.4 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03345-1

  Cytometric bead array standard curve of MP03345-1, GRB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88060-1-PBS. Detection antibody: 88060-2-PBS. Standard: Ag0395. Range: 0.312-40 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88060-1-PBS targets GRB2 as part of a matched antibody pair:

MP03345-1: 88060-1-PBS capture and 88060-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0395

  Product name: Recombinant human GRB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-214 aa of BC000631

  Sequence: MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVN

• Full Name: growth factor receptor-bound protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC000631
• Gene Symbol: GRB2
• Gene ID (NCBI): 2885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62993
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.