GRB2 Recombinant monoclonal antibody

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• GRB2 Recombinant monoclonal antibody (88060-1-RR)

GRB2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88060-1-RR

Clone No.260129D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ASH, EGFRBP GRB2, Grb33, Growth factor receptor-bound protein 2

• 

  WB analysis using 88060-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88060-1-RR (GRB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, Jurkat cells, Raji cells, HEK-293 cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88060-1-RR targets GRB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0395

  Product name: Recombinant human GRB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-214 aa of BC000631

  Sequence: MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVN

• Full Name: growth factor receptor-bound protein 2
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC000631
• Gene Symbol: GRB2
• Gene ID (NCBI): 2885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62993
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-beta protein precursor. Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.