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GRB7 Polyclonal antibody, PBS Only (10045-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 10045-1-Ig (same clone as 10045-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10045-1-Ig (same clone as 10045-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 10045-1-Ig (same clone as 10045-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 10045-1-Ig (GRB7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
IP experiment of A431 using 10045-1-Ig (same clone as 10045-1-PBS)
IP result of anti-GRB7 (IP:10045-1-Ig, 3ug; Detection:10045-1-Ig 1:500) with A431 cells lysate 3300ug. This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10045-1-Ig (same clone as 10045-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10045-1-Ig (GRB7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 10045-1-Ig (same clone as 10045-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GRB7 antibody (10045-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10045-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GRB7 Polyclonal antibody, PBS Only

Name: GRB7 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10045-1-PBS

GRB7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10045-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10045-1-PBS targets GRB7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0023 Product name: Recombinant human GRB7 protein Source: e coli.-derived, Unknow Tag: N/A Domain: Gst-KG-Grb7 of BC006535 Sequence: Predict reactive species
Full Name	growth factor receptor-bound protein 7
Calculated Molecular Weight	532 aa, 60 kDa
Observed Molecular Weight	60-70 kDa
GenBank Accession Number	BC006535
Gene Symbol	GRB7
Gene ID (NCBI)	2886
RRID	AB_2232601
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q14451
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Grb7 belongs to a family of adapter proteins. This family, including Grb10, Grb7 and Grb 14, contains a praline-rich, a RA, a PH, a BPS and SH2 domain. Grb7 associate with a number of receptors including EGFR and FAK. Functions of Grb7 include mobility because it associates receptors such FAK, and direct evidence from overexpression of this proteins.This antibody recognize the N-terminal of GRB7.