GRIN1 Monoclonal antibody
GRIN1 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Rabbit, Chicken and More (1)
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H2C2
Cat no : 67717-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue |
Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
67717-1-Ig targets GRIN1 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.
Tested Reactivity | Human, Mouse, Rat, Rabbit, Chicken |
Cited Reactivity | mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | GRIN1 fusion protein Ag26364 |
Full Name | glutamate receptor, ionotropic, N-methyl D-aspartate 1 |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 105120 kDa |
GenBank Accession Number | NM_000832 |
Gene Symbol | GRIN1 |
Gene ID (NCBI) | 2902 |
RRID | AB_2882906 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GRIN1 antibody 67717-1-Ig | Download protocol |
IHC protocol for GRIN1 antibody 67717-1-Ig | Download protocol |
FC protocol for GRIN1 antibody 67717-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Brain Res The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults |