GRIN1 Monoclonal antibody, PBS Only

GRIN1 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2C2

Cat no : 67717-1-PBS

Synonyms

GRIN1, NR1

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Validation Data Gallery

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  WB analysis using 67717-1-Ig (same clone as 67717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67717-1-Ig (GRIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 67717-1-Ig (GRIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (GRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (GRIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 67717-1-Ig (same clone as 67717-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human GRIN1 (67717-1-Ig, Clone:1H2C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67717-1-PBS targets GRIN1 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRIN1 fusion protein Ag26364
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: GRIN1
• Gene ID (NCBI): 2902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.