GRIP1 Polyclonal antibody

GRIP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22398-1-AP

Synonyms

GRIP, GRIP1

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Validation Data Gallery

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  WB analysis using 22398-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22398-1-AP (GRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 22398-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22398-1-AP (GRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22398-1-AP

  IP result of anti-GRIP1 (IP:22398-1-AP, 4ug; Detection:22398-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

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  IHC staining of human kidney using 22398-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22398-1-AP (GRIP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22398-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22398-1-AP (GRIP1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

22398-1-AP targets GRIP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRIP1 fusion protein Ag18071
• Full Name: glutamate receptor interacting protein 1
• Calculated Molecular Weight: 1128 aa, 122 kDa
• Observed Molecular Weight: 130-135 kDa
• GenBank Accession Number: BC115393
• Gene Symbol: GRIP1
• Gene ID (NCBI): 23426
• RRID: AB_2879099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Development; Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells.; Authors - Akira Kinoshita
mouse	WB	Neuropharmacology; Regulation of hippocampal long term depression by Neuroligin 1.; Authors - Rui Dang
mouse	WB	J Nutr Biochem; Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline; Authors - Ping Lu