Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22398-1-AP targets GRIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18071 Product name: Recombinant human GRIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 702-964 aa of BC115393 Sequence: QTDAQSASSPKKFPISSHLSDLGDVEEDSSPAQKPGKLSDMYPSTVPSVDSAVDSWDGSAIDTSYGTEGTSFQASGYNFNTYDWRSPKQRGSLSPVTKPRSQTYPDVGLSYEDWDRSTASGFAGAADSAETEQEENFWSQALEDLETCGQSGILRELEEKADRRVSLRNMTLLATIMSGSTMSLNHEAPTPRSQLGRQASFQERSSSRPHYSQTTRSNTLPSDVGRKSVTLRKMKQEIKEIMSPTPVELHKVTLYKDSDMEDF Predict reactive species |
| Full Name | glutamate receptor interacting protein 1 |
| Calculated Molecular Weight | 1128 aa, 122 kDa |
| Observed Molecular Weight | 130-135 kDa |
| GenBank Accession Number | BC115393 |
| Gene Symbol | GRIP1 |
| Gene ID (NCBI) | 23426 |
| RRID | AB_2879099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3R0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| IP protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| WB protocol for GRIP1 antibody 22398-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRIP1 Polyclonal antibody (Cat# 22398-1-AP) has a total of 5 citations. These appear in J Nutr Biochem, Neuropharmacology, Frontiers in Molecular Neuroscience, Current Issues in Molecular Biology, and Development. The associated research fields span neuroscience—including hippocampal proteomics in obesity, synaptic plasticity, epilepsy mechanisms, and synaptic protein regulation—and developmental biology, specifically neural crest cell-derived eye tissue formation.
| Species | Application | Title |
|---|---|---|
J Nutr Biochem Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline | ||
Front Mol Neurosci Chronic reduction of synaptic proteins in the epileptogenic lesion of patients with hippocampal sclerosis. | ||
Curr Issues Mol Biol The Frmpd3 Protein Regulates Susceptibility to Epilepsy by Combining with GRIP and GluA2 | ||
Development Itpr1 regulates the formation of anterior eye segment tissues derived from neural crest cells. |
