mGluR1 Polyclonal antibody

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  IHC staining of mouse brain using 19955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19955-1-AP (mGluR1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 19955-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19955-1-AP (mGluR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

mGluR1 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 19955-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

GRM1, MGLUR1A, mGlu1, GRM1A, GPRC1A

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19955-1-AP targets mGluR1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: glutamate receptor, metabotropic 1
• Calculated Molecular Weight: 132 kDa
• GenBank Accession Number: NM_000838
• Gene Symbol: GRM1
• Gene ID (NCBI): 2911
• RRID: AB_10638312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13255
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRM1, also named as GPRC1A and MGLUR1, belongs to the G-protein coupled receptor 3 family. GRM1 is a receptor for glutamate. The activity of GRM1 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It may participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. The antibody can recognize isoform1 and isoform2 of GRM1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Clin Cancer Res; Serum glutamate levels correlate with Gleason score and glutamate blockade decreases proliferation, migration, and invasion and induces apoptosis in prostate cancer cells.; Authors - Koochekpour Shahriar S
human		Int J Biol Sci; Glutamate signaling in benign and malignant disorders: current status, future perspectives, and therapeutic implications.; Authors - Stacey S Willard
human		Brain Commun; M1 muscarinic acetylcholine receptor dysfunction in moderate Alzheimer's disease pathology.; Authors - Jee Hyun Yi