GRP94 Monoclonal antibody, PBS Only (Capture)

GRP94 Monoclonal Antibody for WB, IF, FC, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, FC, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G7C7

Cat no : 60012-2-PBS

Synonyms

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

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Validation Data Gallery

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  WB analysis using 60012-2-Ig (same clone as 60012-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60012-2-Ig (GRP94 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50013-1

  Sandwich ELISA standard curve ofMP50013-1, GRP94 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60012-2-PBS. Detection antibody: HRP-conjugated 60012-1-PBS. Standard: Ag1439. Range: 0.156-20 ng/mL..

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  IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60012-2-Ig (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60012-2-Ig (GRP94 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60012-2-Ig (same clone as 60012-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 60012-2-Ig (GRP94 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 60012-2-Ig (same clone as 60012-2-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GRP94 (60012-2-Ig, Clone:4G7C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60012-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60012-2-PBS targets GRP94 as part of a matched antibody pair:

MP50013-1: 60012-2-PBS capture and 60012-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRP94 fusion protein Ag1439
• Full Name: heat shock protein 90kDa beta (Grp94), member 1
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC009195
• Gene Symbol: HSP90B1
• Gene ID (NCBI): 7184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.