Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293T cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human bowen disease tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1200-1:4800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12720-1-AP targets GRPEL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3415 Product name: Recombinant human GRPEL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC024242 Sequence: MAAQCVRLARRSLPALALSLRPSPRLLCTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA Predict reactive species |
| Full Name | GrpE-like 1, mitochondrial (E. coli) |
| Calculated Molecular Weight | 217 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC024242 |
| Gene Symbol | GRPEL1 |
| Gene ID (NCBI) | 80273 |
| RRID | AB_2114331 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAV7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GRPEL1 antibody 12720-1-AP | Download protocol |
| IHC protocol for GRPEL1 antibody 12720-1-AP | Download protocol |
| IP protocol for GRPEL1 antibody 12720-1-AP | Download protocol |
| WB protocol for GRPEL1 antibody 12720-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GRPEL1 Polyclonal antibody (Cat# 12720-1-AP) has 6 citations published in Nature Communications (3), Molecular Cell (1), Cell Death & Disease (1), and EBioMedicine (1). Research fields span mitochondrial protein import and quality control, mitophagy, pancreatic cancer biomarkers, fatty acid synthesis in PDAC, and cell fate regulation during mitotic stress. All cited studies were conducted in human samples using WB or IHC applications.
| Species | Application | Title |
|---|---|---|
Nat Commun Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy | ||
Nat Commun Inference of upstream-mutation and metabolomic-signature causality identifies prognostic biomarkers and therapeutic targets in pancreatic cancer.
| ||
Mol Cell The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism
| ||
Cell Death Dis c-Myc/GRPEL1 maintains fatty acid synthesis via FASN to support PDAC cell proliferation.
| ||
EBioMedicine The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis. |
