GSDMD Monoclonal antibody

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

36

CloneNo.

2E6C7

Cat no : 66387-1-Ig

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1

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Validation Data Gallery

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  WB analysis using 66387-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66387-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 66387-1-Ig

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 66387-1-Ig

  human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66387-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66387-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 66387-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of TF-1 using 66387-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66387-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of EC109 using 66387-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 66387-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66387-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of THP-1 using 66387-1-Ig

  IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug.

• 

  IF Staining of HeLa using 66387-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66387-1-Ig(GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, COLO 320 cells, EC109 cells, HL-60 cells, human colon tissue, human small intestine tissue, K-562 cells, Raji cells, TF-1 cells, PC-3 cells, U2OS cells, A549 cells, HepG2 cells, Jurkat cells
• Positive IP detected in: THP-1 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 32 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Product Information

66387-1-Ig targets GSDMD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSDMD fusion protein Ag16827
• Full Name: gasdermin D
• Calculated Molecular Weight: 484 aa, 53 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC008904
• Gene Symbol: GSDMD
• Gene ID (NCBI): 79792
• RRID: AB_2881763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Publications

Species	Application	Title
mouse	WB	Mol Cancer; Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases; Authors - Qiang Wang
human	WB	PLoS Pathog; An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).; Authors - Lan Lin
human	WB	iScience; ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1; Authors - Ozlem Calbay
mouse	WB	Biochem Pharmacol; Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice; Authors - Zejin Liu
human	WB	Emerg Microbes Infect; Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.; Authors - Lei Xu
mouse	WB	Mol Med; MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription; Authors - Nan Zhao