Filter: AllWBIPIF/ICC

WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 66387-1-Ig (same clone as 66387-1-PBS)
A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of EC109 using 66387-1-Ig (same clone as 66387-1-PBS)
EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 66387-1-Ig (same clone as 66387-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of human small intestine using 66387-1-Ig (same clone as 66387-1-PBS)
human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of human colon using 66387-1-Ig (same clone as 66387-1-PBS)
human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 66387-1-Ig (same clone as 66387-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of K-562 using 66387-1-Ig (same clone as 66387-1-PBS)
K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 66387-1-Ig (same clone as 66387-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
WB analysis of TF-1 using 66387-1-Ig (same clone as 66387-1-PBS)
TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)
IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (66387-1-Ig, Clone: 2E6C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

GSDMD Monoclonal antibody, PBS Only

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 66387-1-PBS

Clone No.2E6C7

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66387-1-PBS targets GSDMD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag16827 Product name: Recombinant human GSDMD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 135-484 aa of BC008904 Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH Predict reactive species
Full Name	gasdermin D
Calculated Molecular Weight	484 aa, 53 kDa
Observed Molecular Weight	47-53 kDa
GenBank Accession Number	BC008904
Gene Symbol	GSDMD
Gene ID (NCBI)	79792
RRID	AB_2881763
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P57764
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.