GSNOR,ADH5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16379-1-AP

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

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Validation Data Gallery

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  WB analysis of mouse liver using 16379-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16379-1-AP

  WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

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  WB analysis of human liver using 16379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16379-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16379-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16379-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16379-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16379-1-AP

  IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 3ug; Detection:16379-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human endometrial cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).

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  IHC staining of human testis using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16379-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 16379-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GSNOR,ADH5 antibody (16379-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human brain tissue, human testis tissue, mouse heart tissue, HepG2 cells, mouse brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

16379-1-AP targets GSNOR,ADH5 in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSNOR,ADH5 fusion protein Ag9360
• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 374 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014665
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_2289298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.; Authors - Michele Pietrafesa ; KD Validated