GSNOR,ADH5 Polyclonal antibody

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  WB analysis of HepG2 using 16379-1-AP

  WB result of GSNOR,ADH5 antibody (16379-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells.

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  WB analysis using 16379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16379-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16379-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16379-1-AP

  IP result of anti-GSNOR,ADH5 (IP:16379-1-AP, 4ug; Detection:16379-1-AP 1:5000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of human testis using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 16379-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 16379-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HepG2 using 16379-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSNOR,ADH5 antibody (16379-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 16379-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GSNOR,ADH5 Polyclonal antibody (16379-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 16379-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ADH5, ADH 3, ADH 5, ADH3, ADHX

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, human liver tissue, human testis tissue, mouse liver tissue, rat liver tissue, L02 cells
• Positive IP detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

16379-1-AP targets GSNOR,ADH5 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9360

  Product name: Recombinant human GSNOR,ADH5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-374 aa of BC014665

  Sequence: MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI

• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 374 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014665
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_2289298
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11766
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; Gene Copy Number and Post-Transductional Mechanisms Regulate TRAP1 Expression in Human Colorectal Carcinomas.; Authors - Michele Pietrafesa ; KD Validated