GSS Polyclonal antibody

GSS Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, hamster, canine

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15712-1-AP

Synonyms

Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS



Tested Applications

Positive WB detected inCHO cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 9 publications below

Product Information

15712-1-AP targets GSS in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig, hamster, canine samples.

Tested Reactivity human, mouse, rat, pig, hamster, canine
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSS fusion protein Ag8338
Full Name glutathione synthetase
Calculated molecular weight 474 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007927
Gene symbol GSS
Gene ID (NCBI) 2937
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSS antibody 15712-1-APDownload protocol
IHC protocol for GSS antibody 15712-1-APDownload protocol
IF protocol for GSS antibody 15712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
humanWB

Oxid Med Cell Longev

IMCA Induces Ferroptosis Mediated by SLC7A11 through the AMPK/mTOR Pathway in Colorectal Cancer.

Authors - Lei Zhang
ratWB

J Mol Med (Berl)

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Authors - Li Peng
humanWB

Int J Mol Med

Protective effect of naringin against the LPS-induced apoptosis of PC12 cells: Implications for the treatment of neurodegenerative disorders.

Authors - Hui Wang
mouseWB

Sci Rep

Potent effects of dioscin against obesity in mice.

Authors - Min Liu
ratWB

Drug Metab Dispos

Exposure of Rats to Multiple Oral Doses of Dichloroacetate Results in Upregulation of Hepatic Glutathione Transferases and NAD(P)H Dehydrogenase [Quinone] 1.

Authors - Edwin J Squirewell