GSTA1 Monoclonal antibody

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  WB analysis of pig liver using 66624-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 66624-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66624-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66624-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 66624-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse hepatocytes using 66624-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 66624-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66624-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66624-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

GSTA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66624-1-Ig

Clone No.1E1F4

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

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Tested Applications

• Positive WB detected in: pig liver tissue, human liver tissue, rat kidney tissue, mouse hepatocytes, human testis tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66624-1-Ig targets GSTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6084

  Product name: Recombinant human GSTA1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-222 aa of BC053578

  Sequence: MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF

• Full Name: glutathione S-transferase alpha 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC053578
• Gene Symbol: GSTA1
• Gene ID (NCBI): 2938
• RRID: AB_2881984
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08263
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	J Anim Physiol Anim Nutr (Berl); Tolerable upper intake level of iron damages the liver of weaned piglets.; Authors - Haoxuan Ding
human,mouse	WB	Int J Mol Sci; Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1; Authors - Jing Jiang