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GSTK1 Polyclonal antibody, PBS Only (14535-1-PBS)

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WB analysis of HepG2 using 14535-1-AP (same clone as 14535-1-PBS)
WB result of GSTK1 antibody (14535-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
WB analysis using 14535-1-AP (same clone as 14535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
WB analysis using 14535-1-AP (same clone as 14535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
WB analysis using 14535-1-AP (same clone as 14535-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14535-1-AP (GSTK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
IP experiment of Raji using 14535-1-AP (same clone as 14535-1-PBS)
IP result of anti-GSTK1 (IP:14535-1-AP, 3ug; Detection:14535-1-AP 1:1000) with Raji cells lysate 2000ug. This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14535-1-AP (same clone as 14535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14535-1-AP (GSTK1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14535-1-AP (same clone as 14535-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14535-1-AP (GSTK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14535-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GSTK1 Polyclonal antibody, PBS Only

Name: GSTK1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14535-1-PBS

GSTK1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14535-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14535-1-PBS targets GSTK1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6036 Product name: Recombinant human GSTK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC050715 Sequence: MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL Predict reactive species
Full Name	glutathione S-transferase kappa 1
Calculated Molecular Weight	32 kDa
Observed Molecular Weight	27 kDa
GenBank Accession Number	BC050715
Gene Symbol	GSTK1
Gene ID (NCBI)	373156
RRID	AB_2115910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2Q3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity in mice and human, hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).