Tested Applications
| Positive WB detected in | HuH-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HCT 116 cells, Caco-2 cells, SCaBER cells, MCF-10A cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66715-1-Ig targets GSTP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8880 Product name: Recombinant human GSTP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-210 aa of BC010915 Sequence: MPPYTVVYFPVRGRCAALRMLLADQGQSWKEEVVTVETWQEGSLKASCLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYVSLIYTNYEAGKDDYVKALPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGCLDAFPLLSAYVGRLSARPKLKAFLASPEYVNLPINGNGKQ Predict reactive species |
| Full Name | glutathione S-transferase pi 1 |
| Calculated Molecular Weight | 210 aa, 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC010915 |
| Gene Symbol | GSTP1 |
| Gene ID (NCBI) | 2950 |
| RRID | AB_2882066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P09211 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| IHC protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| IP protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| WB protocol for GSTP1 antibody 66715-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSTP1 antibody (Cat# 66715-1-Ig) has 8 total citations published in journals including Phytomedicine, Free Radical Biology and Medicine, Biochemical Pharmacology, Cell & Molecular Life Sciences, European Journal of Pharmacology, Bioorganic Chemistry, Molecular Medicine Reports, and Oncotargets and Therapy. Research fields span cancer biology (renal cell carcinoma, ovarian cancer, esophageal squamous cell carcinoma, glioma), multidrug resistance, ferroptosis, mitochondrial protection, and autophagy regulation.
| Species | Application | Title |
|---|---|---|
Phytomedicine Natural small-molecules reverse Xeroderma Pigmentosum Complementation Group C (XPC) deficient-mediated drug-resistance in renal cell carcinoma | ||
Free Radic Biol Med Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1 | ||
Cell Mol Life Sci NUMA1 modulates apoptosis of esophageal squamous cell carcinoma cells through regulating ASK1-JNK signaling pathway
| ||
Eur J Pharmacol N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis | ||
Bioorg Chem Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1 |
