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  • KD/KO Validated

GSTP1 Monoclonal antibody

GSTP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat no : 66715-1-Ig

Synonyms

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI



Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66715-1-Ig targets GSTP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTP1 fusion protein Ag8880
Full Name glutathione S-transferase pi 1
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC010915
Gene symbol GSTP1
Gene ID (NCBI) 2950
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTP1 antibody 66715-1-IgDownload protocol
IHC protocol for GSTP1 antibody 66715-1-IgDownload protocol
IF protocol for GSTP1 antibody 66715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

PMID: 32535103

Authors - Qi Feng
  • KD Validated
humanWB

Bioorg Chem

Sesquiterpene lactones from Elephantopus scaber exhibit cytotoxic effects on glioma cells by targeting GSTP1

Authors - Qiu-Lin Yan
ratWB

Free Radic Biol Med

Ginsenoside Rg1 protects cardiac mitochondrial function via targeting GSTP1 to block S-glutathionylation of optic atrophy 1

Authors - Huimin Zhu
humanWB

Cell Mol Life Sci

NUMA1 modulates apoptosis of esophageal squamous cell carcinoma cells through regulating ASK1-JNK signaling pathway

Authors - Shuying Yin
  • KD Validated