GTDC1 Monoclonal antibody

GTDC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C4C10

Cat no : 66578-1-Ig

Synonyms

GTDC1, mat Xa

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Validation Data Gallery

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  WB analysis using 66578-1-Ig

  Various tissue were subjected to SDS PAGE followed by western blot with 66578-1-Ig (GTDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66578-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66578-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66578-1-Ig (GTDC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 66578-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 66578-1-Ig (GTDC1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human cerebellum tissue, pig brain tissue, pig cerebellum tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66578-1-Ig targets GTDC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GTDC1 fusion protein Ag9934
• Full Name: glycosyltransferase-like domain containing 1
• Calculated Molecular Weight: 292 aa, 34 kDa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC061699
• Gene Symbol: GTDC1
• Gene ID (NCBI): 79712
• RRID: AB_2881938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.