GTF2H2C Polyclonal antibody

GTF2H2C Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17959-1-AP

Synonyms

GTF2H2C, GTF2H2D

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Validation Data Gallery

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  WB analysis using 17959-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17959-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17959-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17959-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17959-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17959-1-AP (GTF2H2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 17959-1-AP

  IP result of anti-GTF2H2C (IP:17959-1-AP, 4ug; Detection:17959-1-AP 1:500) with HepG2 cells lysate 2240 ug.

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17959-1-AP targets GTF2H2C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF2H2C fusion protein Ag12498
• Full Name: general transcription factor IIH, polypeptide 2C
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC105980
• Gene Symbol: GTF2H2C
• Gene ID (NCBI): 728340
• RRID: AB_2114793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.