GTPBP2 Polyclonal antibody

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• GTPBP2 Polyclonal antibody (11557-1-AP)

GTPBP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11557-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GTP binding protein 2, GTPBP2

• 

  WB analysis of human kidney using 11557-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human spleen using 11557-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11557-1-AP (GTPBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse spleen using 11557-1-AP

  IP result of anti-GTPBP2 (IP:11557-1-AP, 4ug; Detection:11557-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11557-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 11557-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11557-1-AP (GTPBP2 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human kidney tissue, human spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11557-1-AP targets GTPBP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2113

  Product name: Recombinant human GTPBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 183-532 aa of BC028347

  Sequence: SKMITFIDLAGHHKYLHTTIFGLTSYCPDCALLLVSANTGIAGTTREHLGLALALKVPFFIVVSKIDLCAKTTVERTVRQLERVLKQPGCHKVPMLVTSEDDAVTAAQQFAQSPNVTPIFTLSSVSGESLDLLKVFLNILPPLTNSKEQEELMQQLTEFQVDEIYTVPEVGTVVGGTLSSGICREGDQLVVGPTDDGCFLELRVCSIQRNRSACRVLRAGQAATLALGDFDRALLRKGMVMLSPEMNPTICSVFEAEIVLLFHATTFRRGFQVTVHVGNVRQTAVVEKIHAKDKLRTGEKAVVRFRFLKHPEYLKVGAKLLFREGVTKGIGHVTDVQAITAGEAQANMGF

• Full Name: GTP binding protein 2
• Calculated Molecular Weight: 602 aa, 66 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC028347
• Gene Symbol: GTPBP2
• Gene ID (NCBI): 54676
• RRID: AB_2115381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BX10
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Cancer Cell Int; Novel function of GTPBP2 in promoting hepatocellular carcinoma progression through inhibition of BTRC-mediated KRAS degradation.; Authors - Po-Shuan Huang
human	WB	MedComm (2020); Plumbagin Triggers Cuproptosis in Hepatocellular Carcinoma (HCC) via the DNA-Methyltransferase 1 (DNMT1)/microRNA-302a-3p (miR-302a-3p)/ATPase Copper Transporting Beta (ATP7B) Axis; Authors - Chuyu Wang
human	WB,IF	Sci Rep; GTPBP2 inactivates Hippo signaling to promote triple-negative breast cancer cell malignancy.; Authors - Xian Zhao ; KD Validated