GTSE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GTSE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 21319-1-AP

Synonyms

B99, G 2 and S phase expressed 1, GTSE 1, GTSE1, Protein B99 homolog

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Validation Data Gallery

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  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21319-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse spleen using 21319-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, MCF-7 cells, mouse liver tissue, K-562 cells, L02 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

21319-1-AP targets GTSE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTSE1 fusion protein Ag13497
• Full Name: G-2 and S-phase expressed 1
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 66-77 kDa, 100 kDa
• GenBank Accession Number: BC006325
• Gene Symbol: GTSE1
• Gene ID (NCBI): 51512
• RRID: AB_10733644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

Publications

Species	Application	Title
human	IHC	Mod Pathol; Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets.; Authors - Ellinor Andersson
human	WB	Mol Ther Nucleic Acids; lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation.; Authors - Xuelei Ruan
human	IHC	Cancer Sci; High G2 and S-phase expressed 1 expression promotes acral melanoma progression and correlates with poor clinical prognosis.; Authors - Tianxiao Xu
human	WB,IHC	Lab Invest; GTSE1 promotes tumor growth and metastasis by attenuating of KLF4 expression in clear cell renal cell carcinoma.; Authors - Weihao Chen ; KD Validated
human,mouse	WB,IHC	Lab Invest; GTSE1 promotes prostate cancer cell proliferation via the SP1/FOXM1 signaling pathway.; Authors - Wenjie Lai ; KD Validated
human	WB,IHC,IF	Mol Carcinog; G2 and S phase-expressed-1 induces chromosomal instability in esophageal squamous cell carcinoma cells and inhibits cell apoptosis through ROS/JNK signaling; Authors - Man Zhang ; KD Validated