GTSE1 Polyclonal antibody, PBS Only

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• GTSE1 Polyclonal antibody, PBS Only (21319-1-PBS)

• 

  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21319-1-AP (same clone as 21319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21319-1-AP (same clone as 21319-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21319-1-AP (GTSE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 21319-1-AP (same clone as 21319-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21319-1-AP (GTSE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21319-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GTSE1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21319-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

21319-1-PBS targets GTSE1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13497

  Product name: Recombinant human GTSE1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 416-720 aa of BC006325

  Sequence: CAWSESSQLNKTRSIRRRDSCLNSKTKVMPTPTNQFKIPKFSIGDSPDSSTPKLSRAQRPQSCTSVGRVTVHSTPVRRSSGPAPQSLLSARRVSALPTPASRRCSGLPPMTPKTMPRAVGSPLCVPARRRSSEPRKNSAMRTEPTRESNRKTDSRLVDVSPDRGSPPSRVPQALNFSPEESDSTFSKSTATEVAREEAKPGGDAAPSEALLVDIKLEPLAVTPDAASQPLIDLPLIDFCDTPEAHVAVGSESRPLIDLMTNTPDMNKNVAKPSPVVGQLIDLSSPLIQLSPEADKENVDSPLLKF

• Full Name: G-2 and S-phase expressed 1
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 66-77 kDa, 100 kDa
• GenBank Accession Number: BC006325
• Gene Symbol: GTSE1
• Gene ID (NCBI): 51512
• RRID: AB_10733644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYZ3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.