GUCY1B3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GUCY1B3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19011-1-AP

Synonyms

GCS-beta-1, GCS beta 3, GCS beta 1, GC SB3, GC S beta 1

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Validation Data Gallery

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  WB analysis using 19011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 19011-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19011-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19011-1-AP (GUCY1B3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 19011-1-AP

  IP result of anti-GUCY1B3 (IP:19011-1-AP, 4ug; Detection:19011-1-AP 1:500) with human placenta tissue lysate 2600 ug.

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 19011-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19011-1-AP (GUCY1B3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19011-1-AP

  Immunofluorescent analysis of Hela cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 19011-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GUCY1B3 antibody 19011-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse lung tissue, rat lung tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: mouse brain tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

19011-1-AP targets GUCY1B3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GUCY1B3 fusion protein Ag5196
• Full Name: guanylate cyclase 1, soluble, beta 3
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 67-71 kDa
• GenBank Accession Number: BC047620
• Gene Symbol: GUCY1B3
• Gene ID (NCBI): 2983
• RRID: AB_2115817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Aortic disease in Marfan syndrome is caused by overactivation of sGC-PRKG signaling by NO.; Authors - Andrea de la Fuente-Alonso
mouse	WB	J Ethnopharmacol; Modified Zhenwu Decoction improved intestinal barrier function of experimental colitis through activation of sGC-mediated cGMP/PKG signaling; Authors - Yiqi Xu
mouse	WB,IHC	Clin Sci (Lond); Empagliflozin prevents cardiomyopathy via sGC-cGMP-PKG pathway in type 2 diabetes mice.; Authors - Mei Xue ; KD Validated
mouse	WB,IHC	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	Cell Tissue Res; Cavin-3 (PRKCDBP) deficiency reduces the density of caveolae in smooth muscle.; Authors - Baoyi Zhu
rat	WB	World Neurosurg; Upregulation of Connexin 40 Mediated by Nitric Oxide Attenuates Cerebral Vasospasm After Subarachnoid Hemorrhage via the Nitric Oxide-Cyclic Guanosine Monophosphate-Protein Kinase G Pathway.; Authors - Shi Hai Lan