GUCY2F Polyclonal antibody

GUCY2F Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25252-1-AP

Synonyms

CYGF, GC F, guanylate cyclase 2F, retinal, Guanylate cyclase F, GUC2DL, GUC2F, GUCY2F, RETGC 2, RETGC2, Retinal guanylyl cyclase 2, ROS GC2

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Validation Data Gallery

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  WB analysis of mouse heart using 25252-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25252-1-AP (GUCY2F Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 25252-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25252-1-AP (GUCY2F Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25252-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25252-1-AP (GUCY2F Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

The immunogen of 25252-1-AP is GUCY2F Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GUCY2F fusion protein Ag19266
• Full Name: guanylate cyclase 2F, retinal
• Calculated Molecular Weight: 1108 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC156674
• Gene Symbol: GUCY2F
• Gene ID (NCBI): 2986
• RRID: AB_2879989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GUCY2F(Guanylate cyclase 2F, retinal) is also named as GUC2F, RETGC2 and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. GUCY2F may play a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. By in situ hybridization, mRNA encoding GUCY2F is found only in the outer nuclear layer and inner segments of photoreceptor cells. By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2(GUCY2F) can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments.(PMID:7777544).

Publications

Species	Application	Title
human	IF	iScience; A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.; Authors - Suguru Yamasaki
mouse	IF	Transl Vis Sci Technol; Construction and Identification of a Novel Mice Model of Microphthalmia; Authors - Dan Li