GUF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17489-1-AP

Synonyms

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 17489-1-AP

  WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

• 

  WB analysis using 17489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17489-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 17489-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17489-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17489-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17489-1-AP

  IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 17489-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17489-1-AP

  Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, mouse pancreas tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human oesophagus tissue, human lung cancer tissue, human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17489-1-AP targets GUF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GUF1 fusion protein Ag11579
• Full Name: GUF1 GTPase homolog (S. cerevisiae)
• Calculated Molecular Weight: 669 aa, 74 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC036768
• Gene Symbol: GUF1
• Gene ID (NCBI): 60558
• RRID: AB_2115960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.